Targeting the expression of functional murine CMP-sialic acid transporter to the E. coli inner membrane.

@article{Maggioni2007TargetingTE,
  title={Targeting the expression of functional murine CMP-sialic acid transporter to the E. coli inner membrane.},
  author={Andrea Maggioni and Mark von Itzstein and Rita Gerardy-Schahn and Joe Tiralongo},
  journal={Biochemical and biophysical research communications},
  year={2007},
  volume={362 3},
  pages={779-84}
}
The heterologous expression of functional mammalian integral membrane proteins still represents a significant hurdle towards their crystallization and structure elucidation. We have therefore explored the use of the OmpA signal sequence to deliberately target the expression of the murine CMP-sialic acid transporter, a Golgi-resident protein with 10 putative transmembrane domains, to the Escherichia coli inner membrane. Here, we show that the expression of an OmpA signal sequence-FLAG-CMP-sialic… CONTINUE READING