Targeting the Bacillus subtilis genome: an efficient and clean method for gene disruption.


A method to disrupt multiple Bacillus subtilis genes is described. A resistance cassette is used to interrupt an amplified target sequence from the B. subtilis chromosome. The cassette is composed of a gene conferring resistance to chloramphenicol (Cm) or spectinomycin (Sp) flanked by two directly oriented beta cognate sites (six site) (SCS or SSS… (More)


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