Targeting pyruvate kinase M2 contributes to radiosensitivity of non-small cell lung cancer cells in vitro and in vivo.

@article{Meng2015TargetingPK,
  title={Targeting pyruvate kinase M2 contributes to radiosensitivity of non-small cell lung cancer cells in vitro and in vivo.},
  author={Mao-Bin Meng and Huan-huan Wang and Wen-hao Guo and Zhiqiang Wu and Xian-Liang Zeng and Nicholas G Zaorsky and Huashan Shi and Dong Qian and Zhi-Min Niu and Bo Jiang and Lu-jun Zhao and Zhiyong Yuan and Ping F. Wang},
  journal={Cancer letters},
  year={2015},
  volume={356 2 Pt B},
  pages={985-93}
}
Aerobic glycolysis, a metabolic hallmark of cancer, is associated with radioresistance in non-small cell lung cancer (NSCLC). Pyruvate kinase M2 isoform (PKM2), a key regulator of glycolysis, is expressed exclusively in cancers. However, the impact of PKM2 silencing on the radiosensitivity of NSCLC has not been explored. Here, we show a plasmid of shRNA-PKM2 for expressing a short hairpin RNA targeting PKM2 (pshRNA-PKM2) and demonstrate that treatment with pshRNA-PKM2 effectively inhibits PKM2… CONTINUE READING