Targeting of hESCs and hiPSCs using TALEN mediated homologous recombination hESCs and hiPSCs were cultured in Rho Kinase (ROCK)-inhibitor (Calbiochem;

  • Published 2011

Abstract

Cell culture Cell culture techniques have been described previously. C1 hiPSCs and the hESC lines WIBR#3 were maintained on mitomycin C inactivated mouse embryonic fibroblast (MEF) feeder layers in hESC medium [DMEM/F12 (Invitrogen) supplemented with 15% fetal bovine serum (FBS) (Hyclone), 5% KnockOutTM Serum Replacement (Invitrogen), 1 mM glutamine… (More)

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