Targeting of biotinylated compounds to its target tissue using a low-density lipoprotein receptor–avidin fusion protein


The very high binding affinity of avidin to biotin is one of the highest to occur in nature. We constructed a fusion protein composed of avidin and the endocytotic LDL receptor in order to target biotinylated molecules to cells of the desired tissues. In addition to the native avidin, charge-mutated and nonglycosylated avidins were utilized as part of the… (More)
DOI: 10.1038/


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