Targeting of antisense DNA: comparison of activity of anti-rabbit beta-globin oligodeoxyribonucleoside phosphorothioates with computer predictions of mRNA folding.

@article{Jaroszewski1993TargetingOA,
  title={Targeting of antisense DNA: comparison of activity of anti-rabbit beta-globin oligodeoxyribonucleoside phosphorothioates with computer predictions of mRNA folding.},
  author={Jerzy W. Jaroszewski and J L Syi and Mrinal Kanti Ghosh and Kaushik Ghosh and Jack S. Cohen},
  journal={Antisense research and development},
  year={1993},
  volume={3 4},
  pages={339-48}
}
To assess the usefulness of computer-assisted modeling of mRNA as an aid in design of antisense DNA, the efficiency of inhibition of translation of rabbit beta-globin mRNA by various antisense sequences was compared with calculated structures of the mRNA. The model obtained by consideration of 30 lowest-energy computer-simulated structures is consistent with the high accessibility of the AUG initiation codon region known from digestion with nucleases and with previous antisense inhibition… CONTINUE READING