Targeting of a histone acetyltransferase domain to a promoter enhances protein expression levels in mammalian cells.

@article{Kwaks2005TargetingOA,
  title={Targeting of a histone acetyltransferase domain to a promoter enhances protein expression levels in mammalian cells.},
  author={Ted H. J. Kwaks and Richard G. A. B. Sewalt and Rik van Blokland and Tjalling Siersma and Mobien M A Kasiem and Ang{\`e}le Kelder and Arie P. Otte},
  journal={Journal of biotechnology},
  year={2005},
  volume={115 1},
  pages={35-46}
}
Silencing of transfected genes in mammalian cells is a fundamental problem that probably involves the (in)accessibility status of chromatin. A potential solution to this problem is to provide a cell with protein factors that make the chromatin of a promoter more open or accessible for transcription. We tested this by targeting such proteins to different promoters. We found that targeting the p300 histone acetyltransferase (HAT) domain to strong viral or cellular promoters is sufficient to… CONTINUE READING
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