Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae.

@article{Schreuder1993TargetingOA,
  title={Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae.},
  author={Maartje Schreuder and Stephan Brekelmans and Herman van den Ende and Frans Klis},
  journal={Yeast},
  year={1993},
  volume={9 4},
  pages={399-409}
}
The sexual adhesion protein of Saccharomyces cerevisiae MAT alpha cells, alpha-agglutinin, could not be extracted from the cell wall with hot sodium dodecyl sulfate (SDS), but became soluble after digestion of the cell wall with laminarinase. This indicates that it is intimately associated with cell wall glucan. A fusion protein was constructed consisting of the signal sequence of yeast invertase, guar alpha-galactosidase, and the C-terminal half of the alpha-agglutinin. Most of the fusion… CONTINUE READING