Targeting of Cameleons to various subcellular compartments reveals a strict cytoplasmic/mitochondrial Ca²⁺ handling relationship in plant cells.

@article{Loro2012TargetingOC,
  title={Targeting of Cameleons to various subcellular compartments reveals a strict cytoplasmic/mitochondrial Ca²⁺ handling relationship in plant cells.},
  author={Giovanna Loro and Ilaria Drago and Tullio Pozzan and F. Ricciardi Lo Schiavo and Michela Zottini and Alex Costa},
  journal={The Plant journal : for cell and molecular biology},
  year={2012},
  volume={71 1},
  pages={
          1-13
        }
}
Here we describe use of a mitochondrial targeted Cameleon to produce stably transformed Arabidopsis plants that enable analyses of mitochondrial Ca²⁺ dynamics in planta and allow monitoring of the intra-mitochondrial Ca²⁺ concentration in response to physiological or environmental stimuli. Transgenic plants co-expressing nuclear and mitochondrial targeted Cameleons were also generated and analyzed. Here we show that mitochondrial Ca²⁺ accumulation is strictly related to the intensity of the… CONTINUE READING
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