Targeting neural circuitry in zebrafish using GAL4 enhancer trapping


We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.

DOI: 10.1038/nmeth1033

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@article{Scott2007TargetingNC, title={Targeting neural circuitry in zebrafish using GAL4 enhancer trapping}, author={Ethan K. Scott and Lindsay Mason and Aristides B Arrenberg and Limor Ziv and Nathan J. Gosse and Tong Xiao and Neil C Chi and Kazuhide Asakawa and Koichi Kawakami and Herwig Baier}, journal={Nature Methods}, year={2007}, volume={4}, pages={323-326} }