Targeting ie‐1 gene by RNAi induces baculoviral resistance in lepidopteran cell lines and in transgenic silkworms

@article{Kanginakudru2007TargetingIG,
  title={Targeting ie‐1 gene by RNAi induces baculoviral resistance in lepidopteran cell lines and in transgenic silkworms},
  author={Sriramana Kanginakudru and Corinne Royer and S V Edupalli and Audrey Jalabert and Bernard Mauchamp and Surakasi Venkata Prasad and G{\'e}rard Chavancy and Pierre Couble and Javaregowda Nagaraju},
  journal={Insect Molecular Biology},
  year={2007},
  volume={16}
}
RNA interference (RNAi)‐mediated viral inhibition has been used in a few organisms for eliciting viral resistance. In the present study, we report the use of RNAi in preventing baculovirus infection in a lepidopteran. We targeted the baculoviral immediate early‐1 (ie‐1) gene in both a transformed lepidopteran cell line and in the transgenic silkworm Bombyx mori L. Constitutive expression of double‐stranded RNA was achieved by piggyBac‐mediated transformation of Sf9 cell line with a transgene… 
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Targeting genes involved in nucleopolyhedrovirus DNA multiplication through RNA interference technology to induce resistance against the virus in silkworms
TLDR
The study revealed the successful use of bacteria as vectors for efficiently delivering dsRNA to elicit RNAi against BmNPV in silkworms.
Resistance of transgenic silkworm to BmNPV could be improved by silencing ie-1 and lef-1 genes
TLDR
The use of transgenic RNAi in preventing Bombyx mori nucleopolyhedrovirus multiplication in the transgenic silkworm B. mori is reported, suggesting that double-stranded RNA expression of essential genes of BmNPV is a feasible method for breeding silkworms with a high antiviral capacity.
Over‐expression of RNA interference (RNAi) core machinery improves susceptibility to RNAi in silkworm larvae
TLDR
It is hypothesize that over‐expression of core RNAi machinery enzymes can improve RNAi efficacy in traditionally recalcitrant species, and offers a new strategy for functional genomics research in RNAi‐refractory insect taxa in general and for lepidopterans in particular.
Construction of Baculovirus-Inducible CRISPR/Cas9 Antiviral System Targeting BmNPV in Bombyx mori
The silkworm Bombyx mori is an economically important insect. The sericulture industry is seriously affected by pathogen infections. Of these pathogens, Bombyx mori nucleopolyhedrovirus (BmNPV)…
Inhibition of BmNPV replication in silkworm cells using inducible and regulated artificial microRNA precursors targeting the essential viral gene lef-11.
Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm
TLDR
Examination of the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations indicated co-regulation of NPV gene expression in the presence of transgenes.
Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines
TLDR
It is demonstrated that overexpression of Dicer2 and Argonaute2 in lepidopteran cells leads to improved defense against the acute infection of the Cricket Paralysis Virus, and the potential use of an RNAi gain-of-function approach as an alternative strategy to obtain reduced viral-induced mortality in Lepidoptera.
Comparison of factors that may affect the inhibitory efficacy of transgenic RNAi targeting of baculoviral genes in silkworm, Bombyx mori.
Use of Bombyx mori U6 promoter for inducing gene-silencing in silkworm cells
TLDR
A Bombyx mori U6 promoter (U6p)based short hairpin RNA (shRNA) expression system for inducing RNAi in silkworm cells was developed and the ability of the U6-shRNA unit to confer resistance on BmN cells was demonstrated.
Transgenically mediated shRNAs targeting conserved regions of foot-and-mouth disease virus provide heritable resistance in porcine cell lines and suckling mice
TLDR
The results suggest that these heritable shRNAs were able to suppress FMDV replication in the transgenic cell lines and suckling mice.
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TLDR
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