Targeting cytochrome C oxidase in mitochondria with Pt(II)-porphyrins for photodynamic therapy

@inproceedings{Boersch2010TargetingCC,
  title={Targeting cytochrome C oxidase in mitochondria with Pt(II)-porphyrins for photodynamic therapy},
  author={Michael Boersch},
  booktitle={BiOS},
  year={2010}
}
  • M. Boersch
  • Published in BiOS 4 February 2010
  • Biology, Chemistry
Mitochondria are the power house of living cells, where the synthesis of the chemical "energy currency" adenosine triphosphate (ATP) occurs. Oxidative phosphorylation by a series of membrane protein complexes I to IV, that is, the electron transport chain, is the source of the electrochemical potential difference or proton motive force (PMF) of protons across the inner mitochondrial membrane. The PMF is required for ATP production by complex V of the electron transport chain, i.e. by FoF1-ATP… 
View on SPIE
arxiv.org
11 Citations
Highly Influential Citations
1
Background Citations
2
Methods Citations
1

11 Citations

Targeting Mitochondria by Zn(II)N-Alkylpyridylporphyrins: The Impact of Compound Sub-Mitochondrial Partition on Cell Respiration and Overall Photodynamic Efficacy
TLDR
It is found that subcellular distribution of Zn-porphyrins is directed by the nature of the substituents attached to the meso pyridyl nitrogens at the porphyrin ring, and photoinactivation of critical cellular targets explains the superior efficiency of the hexyl derivative in causing mitochondrial photodamage, and suppressing cellular respiration and survival.
Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy
TLDR
Their mitochondrial nano-environment was investigated by FLIM and superresolution microscopy in living human cells and different lifetimes and anisotropy values were found.
The localization of the photosensitizer determines the dynamics of the secondary production of hydrogen peroxide in cell cytoplasm and mitochondria.
Visualizing Mitochondrial FoF1-ATP Synthase as the Target of the Immunomodulatory Drug Bz-423
TLDR
It is reported how Förster resonance energy transfer can be employed to colocalize the enzyme and the fluorescently tagged Bz-423 within the mitochondria of living cells with nanometer resolution.
Observing single FoF1-ATP synthase at work using an improved fluorescent protein mNeonGreen as FRET donor
TLDR
The novel FRET donor mNeonGreen is evaluated as a fusion to FoF1-ATP synthase and compare it to the previously used fluorophore EGFP to evaluate the biochemical purification procedures and activity measurements of the fully functional mutant enzyme.
Triplet-state dynamics of a metalloporphyrin photosensitiser (PtTMPyP4) in the presence of halides and purine mononucleotides.
  • Páraic M Keane, J. Kelly
  • Chemistry, Physics
    Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
  • 2011
TLDR
No charge-separated photoproducts are observed by transient UV/vis/near-IR absorption spectroscopy on the nanosecond timescale, suggesting that rapid reverse electron transfer may prevent type 1 DNA damage.
Observing conformations of single FoF1-ATP synthases in a fast anti-Brownian electrokinetic trap
TLDR
A version of an ABELtrap with a laser focus pattern generated by electro-optical beam deflectors and controlled by a programmable FPGA is presented, which could hold single fluorescent nanobeads for more than 100 seconds and increase the observation times of a single particle more than 1000-fold.
Effects of phenyl/thienyl substituents at acetylacetone auxiliary ligands on the properties of cyclometalated platinum(II) complexes
Porphyrin-based cationic amphiphilic photosensitisers as potential anticancer, antimicrobial and immunosuppressive agents
TLDR
Porphyrin-based photosensitisers with amphiphilic properties, bearing one or more positive charges, are an effective tool in PDT against cancers, microbial infections and, most recently, autoimmune skin disorders.
Ground and excited state interactions of metalloporphyrin PtTMPyP4 with polynucleotides [poly(dG-dC)]2 and [poly(dA-dT)]2.
  • Páraic M Keane, J. Kelly
  • Biology
    Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
  • 2016
The ground- and excited-state interactions of Pt(ii) meso-tetrakis(4-N-methylpyridyl)porphyrin (PtTMPyP4) with polynucleotides [poly(dG-dC)]2 and [poly(dA-dT)]2 have been investigated using…
...
...

References

SHOWING 1-10 OF 53 REFERENCES
Influence of trans-membrane potential and of hydrophobic interactions on dye accumulation in mitochondria of living cells
TLDR
The influence of hydrophobic interactions was investigated by competitive staining experiments using dyes with very different lipophilic properties and the lipophilicity of the dyes was characterized by their Rm values in reversed phase thin-layer chromatography.
Selective photosensitization of mitochondria by the lipophilic cationic porphyrin POR10.
Transport and accumulation of lipophilic dye cations at the mitochondria of HeLa cells in situ.
TLDR
The vital staining of mitochondrial in HeLa cells was investigated with various cationic styrylindolenines and indocarbocyanines and indicated that the dye molecules were accumulated in strongly lipophilic areas of the mitochondria.
Electron-transfer mechanism of the triplet state quenching of aluminium tetrasulfonated phthalocyanine by cytochrome c.
Binding of single nucleotides to H+-ATP synthases observed by fluorescence resonance energy transfer.
Quantum dots for single-pair fluorescence resonance energy transfer in membrane- integrated EFoF1.
TLDR
TIRFM (total internal reflection microscopy) shows that covalent binding of the QD (quantum dot) via cysteine to FoF1 leads to a significant decrease in the blinking probability in the microsecond-to-second time range.
The Proton-translocating a Subunit of F0F1-ATP Synthase Is Allocated Asymmetrically to the Peripheral Stalk*
TLDR
The position of the a subunit of the membrane-integral F0 sector of Escherichia coli ATP synthase was investigated, finding that this relationship provides stability to the membrane interface between a and b2, allowing it to withstand the torque imparted by the rotor during ATP synthesis as well as ATP hydrolysis.
Photoaffinity labelling with fluorescence detection. Dye accumulation at four mitochondrial proteins in HeLa and LM cells.
TLDR
By combining photoaffinity labelling of cultured cells, micro-gel electrophoresis and detection of the fluorescence with a microfluorimeter, this work established a highly sensitive and rapid procedure to identify APMC labelled proteins.
Subunit movements in membrane-integrated EF0F1 during ATP synthesis detected by single-molecule spectroscopy.
Structural organization of the V-ATPase and its implications for regulatory assembly and disassembly.
TLDR
Reversible assembly/disassembly of V-ATPases has certain conformational constraints, which are best fulfilled by adopting a unique conformation before disassembly, which leads to an efficient shutdown of ATP consumption.
...
...