Targeted transformation in Coprinus cinereus

  title={Targeted transformation in Coprinus cinereus},
  author={David M. Binninger and Landry Le Chevanton and C{\'e}cile Skrzynia and Catherine D. Shubkin and Patricia T. Pukkila},
  journal={Molecular and General Genetics MGG},
We examined the influence of DNA form and size on the arrangement and genomic location of transforming DNA sequences in the basidiomycete Coprinus cinereus. Protoplasts with either single or double mutations in the tryptophan synthetase (TRPI) gene were transformed with cloned copies of this gene which contained only a single DNA strand, contained a specific single nick within the C. cinereus sequences (4.8 kb), contained a specific double-strand break, or contained an additional 35 kb of… CONTINUE READING

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