Targeted transformation in Coprinus cinereus

@article{Binninger1991TargetedTI,
  title={Targeted transformation in Coprinus cinereus},
  author={David M. Binninger and Landry Le Chevanton and C{\'e}cile Skrzynia and Catherine D. Shubkin and Patricia T. Pukkila},
  journal={Molecular and General Genetics MGG},
  year={1991},
  volume={227},
  pages={245-251}
}
We examined the influence of DNA form and size on the arrangement and genomic location of transforming DNA sequences in the basidiomycete Coprinus cinereus. Protoplasts with either single or double mutations in the tryptophan synthetase (TRPI) gene were transformed with cloned copies of this gene which contained only a single DNA strand, contained a specific single nick within the C. cinereus sequences (4.8 kb), contained a specific double-strand break, or contained an additional 35 kb of… CONTINUE READING

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Molecular genetics of the agaric, Coprinus cinereus. In: Bennett JW, Lasure LL (eds) More gene manipulation in fungi

P JPukkila, LAC Casselton
1991

Molecular characterization of TRPI , a gene coding for tryptophan synthetase in the basidiomycete Coprinus cinereus

C Skrzynia, DM Binninger, Alspaugh JAII, PJ Pukkila
Gene • 1989

Transformation in fungi

gincham JRS
Microbiol Rev • 1989

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