Targeted transfection by femtosecond laser.

  title={Targeted transfection by femtosecond laser.},
  author={Uday Krishna Tirlapur and Karsten Koenig},
  volume={418 6895},
The challenge for successful delivery of foreign DNA into cells in vitro, a key technique in cell and molecular biology with important biomedical implications, is to improve transfection efficiency while leaving the cell's architecture intact. Here we show that a variety of mammalian cells can be directly transfected with DNA without perturbing their structure by first creating a tiny, localized perforation in the membrane using ultrashort (femtosecond), high-intensity, near-infrared laser… CONTINUE READING
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