Targeted site-directed mutagenesis of a heme oxygenase locus by gene replacement in the moss Ceratodon purpureus

@article{Brcker2004TargetedSM,
  title={Targeted site-directed mutagenesis of a heme oxygenase locus by gene replacement in the moss Ceratodon purpureus},
  author={Gerhard Br{\"u}cker and Franz Mittmann and E. Hartmann and Tilman Lamparter},
  journal={Planta},
  year={2004},
  volume={220},
  pages={864-874}
}
The moss Physcomitrella patens is so far the only plant species in which it is possible for nuclear genes to be modified by homologous recombination at a reasonably efficiency. Here we describe the use of homologous recombination for another moss, Ceratodon purpureus. Our approach is based on the repair of the ptr116 mutant allele. In this mutant, codon 31 of the heme oxygenase gene CpHO1 is mutated to a stop codon. Heme oxygenase is necessary for the conversion of heme to biliverdin, the… CONTINUE READING
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