Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides

@article{Cavaill1996TargetedRM,
  title={Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides},
  author={J{\'e}r{\^o}me Cavaill{\'e} and Monique Nicoloso and Jean Pierre Bachellerie},
  journal={Nature},
  year={1996},
  volume={383},
  pages={732-735}
}
EUKARYOTIC ribosomal RNAs are post-transcriptionally modified by methylation at the ribose sugar of specific nucleotides1. This takes place in the nucleolus and involves a family of small nucleolar RNAs (snoRNAs) with long regions (10–21 nucleotides) complementary to rRNA sequences spanning the methylation site2–4—a complementary snoRNA is required for methylation at a specific site5. Here we show that altering the sequence of the snoRNA is sufficient to change the specificity of methylation… 
Probing RNA in vivo with methylation guide small nucleolar RNAs.
TLDR
A method is developed for constructing libraries of snoRNA genes that, in principle, can introduce methylation point mutations into any rRNA segment of interest and preliminary results are presented that show the feasibility of using this technology to probe a region of the yeast large subunit rRNA that includes the core of the peptidyltransferase center.
SnoRNA-guided ribose methylation of rRNA: structural features of the guide RNA duplex influencing the extent of the reaction.
TLDR
Overall, the RNA duplex tolerates a much larger degree of irregularity than anticipated, even in the immediate vicinity of the methylation site, which offers new prospects in the search for additional snoRNA guides.
Small Nucleolar RNAs Guide the Ribose Methylations of Eukaryotic rRNAs
This chapter provides a progress report on developments that should set the stage for dissecting the rRNA-ribose methylation machinery and for deciphering the role of these modifications in ribosome
Interference probing of rRNA with snoRNPs: a novel approach for functional mapping of RNA in vivo.
TLDR
It is reported that methylation snoRNPs can be targeted to many new sites in yeast rRNA, by providing the snoRNA with a novel guide sequence, and that in some cases growth and translation activity are strongly impaired.
Small nucleolar RNA‐guided post‐transcriptional modification of cellular RNAs
  • T. Kiss
  • Biology, Chemistry
    The EMBO journal
  • 2001
TLDR
Post‐transcriptional modification of individual ribonucleotides, namely deamination of adenosines and cytidines, can also change the readout of mRNAs, and lack of ribosomal pseudouridines can reduce the growth rate or confer a selective disadvantage when it is competed against wild‐type ribosomes.
Targeted pre-mRNA modification for gene silencing and regulation
TLDR
It is demonstrated that a Box C/D RNA can guide modification at the pre-mRNA branch point, thus silencing its expression and inducing cell death.
Structure and biogenesis of small nucleolar RNAs acting as guides for ribosomal RNA modification.
TLDR
Information is reviewed about the biogenesis, structure and function of guide snoRNAs, which function as guides directing site-specific 2'-O-ribose methylation or pseudouridine formation in eukaryotic cells.
Profiling of RNA ribose methylation in Arabidopsis thaliana
TLDR
The RiboMeth-seq data reveal the comprehensive 2′-O-methylation maps for Arabidopsis r RNAs and snRNAs and would facilitate study of their function and biosynthesis and the role of FIB1 or FIB2 in methylation.
RNA nucleotide methylation
TLDR
This review outlines the different functions of methyl groups in RNA, including biophysical, biochemical and metabolic stabilization of RNA, quality control, resistance to antibiotics, mRNA reading frame maintenance, deciphering of normal and altered genetic code, selenocysteine incorporation, tRNA aminoacylation, ribotoxins, splicing, intracellular trafficking, immune response, and others.
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