Targeted ribonuclease can inhibit replication of hepatitis B virus.

  title={Targeted ribonuclease can inhibit replication of hepatitis B virus.},
  author={Jun Liu and Yinghui Li and Cai-fang Xue and Jin Ding and Wei-dong Gong and Ya Zhao and Yu-xiao Huang},
  journal={World journal of gastroenterology},
  volume={9 2},
AIM To study the effect of a novel targeted ribonuclease (TN), the fusion protein of HBVc and human eosinophil-derived neurotoxin (hEDN), on the HBV replication in vitro. METHODS The gene encoding the targeted ribonuclease was cloned into pcDNA3.1 (-) to form recombinant eukaryotic expression vector p/TN. Control plasmids, including p/hEDN, p/HBVc, and p/TNmut in which a Lys113-Arg mutation was introduced by sequential PCR to eliminate the ribonuclease activity of hEDN, were also constructed… CONTINUE READING