Targeted recovery of mutations in Drosophila.

@article{Bentley2000TargetedRO,
  title={Targeted recovery of mutations in Drosophila.},
  author={Alex Bentley and Bruce MacLennan and Judith Calvo and Charles R. Dearolf},
  journal={Genetics},
  year={2000},
  volume={156 3},
  pages={1169-73}
}
Reverse genetic techniques will be necessary to take full advantage of the genomic sequence data for Drosophila and other experimental organisms. To develop a method for the targeted recovery of mutations, we combined an EMS chemical mutagenesis regimen with mutation detection by denaturing high performance liquid chromatography (DHPLC). We recovered mutant strains at the high rate of approximately 4.8 mutations/kb for every 1000 mutagenized chromosomes from a screen for new mutations in the… CONTINUE READING

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