Targeted proteomic quantification on quadrupole-orbitrap mass spectrometer.

@article{Gallien2012TargetedPQ,
  title={Targeted proteomic quantification on quadrupole-orbitrap mass spectrometer.},
  author={S{\'e}bastien Gallien and Elodie Duriez and Catharina Crone and Markus Kellmann and Thomas Moehring and Bruno Domon},
  journal={Molecular & cellular proteomics : MCP},
  year={2012},
  volume={11 12},
  pages={1709-23}
}
There is an immediate need for improved methods to systematically and precisely quantify large sets of peptides in complex biological samples. To date protein quantification in biological samples has been routinely performed on triple quadrupole instruments operated in selected reaction monitoring mode (SRM), and two major challenges remain. Firstly, the number of peptides to be included in one survey experiment needs to be increased to routinely reach several hundreds, and secondly, the degree… CONTINUE READING