Targeted mutagenesis of duplicated genes in soybean with zinc-finger nucleases.

@article{Curtin2011TargetedMO,
  title={Targeted mutagenesis of duplicated genes in soybean with zinc-finger nucleases.},
  author={Shaun J Curtin and Feng Zhang and Jeffry D. Sander and William J. Haun and Colby Starker and Nicholas J. Baltes and Deepak Reyon and Elizabeth Dahlborg and Mathew J. Goodwin and Andrew P Coffman and Drena Dobbs and J Keith Joung and Daniel F. Voytas and Robert M Stupar},
  journal={Plant physiology},
  year={2011},
  volume={156 2},
  pages={
          466-73
        }
}
We performed targeted mutagenesis of a transgene and nine endogenous soybean (Glycine max) genes using zinc-finger nucleases (ZFNs). A suite of ZFNs were engineered by the recently described context-dependent assembly platform--a rapid, open-source method for generating zinc-finger arrays. Specific ZFNs targeting dicer-like (DCL) genes and other genes involved in RNA silencing were cloned into a vector under an estrogen-inducible promoter. A hairy-root transformation system was employed to… CONTINUE READING

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