Targeted mutagenesis in chicken using CRISPR/Cas9 system.

@article{Oishi2016TargetedMI,
  title={Targeted mutagenesis in chicken using CRISPR/Cas9 system.},
  author={Isao Oishi and Kyoko Yoshii and Daichi Miyahara and Hiroshi Kagami and Takahisa Tagami},
  journal={Scientific reports},
  year={2016},
  volume={6},
  pages={
          23980
        }
}
The CRISPR/Cas9 system is a simple and powerful tool for genome editing in various organisms including livestock animals. However, the system has not been applied to poultry because of the difficulty in accessing their zygotes. Here we report the implementation of CRISPR/Cas9-mediated gene targeting in chickens. Two egg white genes, ovalbumin and ovomucoid, were efficiently (>90%) mutagenized in cultured chicken primordial germ cells (PGCs) by transfection of circular plasmids encoding Cas9, a… CONTINUE READING

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