Targeted mutagenesis by homologous recombination in D. melanogaster.

  title={Targeted mutagenesis by homologous recombination in D. melanogaster.},
  author={Yikang S Rong and Simon W. A. Titen and Heng B. Xie and Mary M. Golic and Michael J. Bastiani and Pradip K. Bandyopadhyay and Baldomero M Olivera and Michael H. Brodsky and Gerald M. Rubin and Kent G. Golic},
  journal={Genes & development},
  volume={16 12},
We used a recently developed method to produce mutant alleles of five endogenous Drosophila genes, including the homolog of the p53 tumor suppressor. Transgenic expression of the FLP site-specific recombinase and the I-SceI endonuclease generates extrachromosomal linear DNA molecules in vivo. These molecules undergo homologous recombination with the corresponding chromosomal locus to generate targeted alterations of the host genome. The results address several questions about the general… CONTINUE READING
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