Targeted integration into the Acremonium chrysogenum genome: disruption of the pcbC gene

@article{Walz1993TargetedII,
  title={Targeted integration into the Acremonium chrysogenum genome: disruption of the pcbC gene},
  author={Markus Walz and Ulrich K{\"u}ck},
  journal={Current Genetics},
  year={1993},
  volume={24},
  pages={421-427}
}
The cephalosporin C-producing fungus Acremonium chrysogenum was transformed to hygromycin B resistance using different vector constructs. These constructs contain sequences of the pcbC gene from A. chrysogenum, encoding isopenicillin N synthetase. Detailed analysis of transformants, including pulsed-field gel electrophoresis (PFGE), suggests that integration of multiple vector copies takes place predominantly via non-homologous integration. By increasing the length of vector-DNA homologous to… CONTINUE READING
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