Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase.

@article{Sauer1990TargetedIO,
  title={Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase.},
  author={Brian Sauer and Nadene Henderson},
  journal={The New biologist},
  year={1990},
  volume={2 5},
  pages={441-9}
}
Cre is a 38-kD protein from bacteriophage P1 that catalyzes site-specific recombination between 34-bp loxP sequences. Our previous work has shown that Cre can perform site-specific excisive recombination not only in prokaryotes, but also in eukaryotes such as yeast and cultured mammalian cells. In this work we show that intermolecular Cre-mediated recombination can specifically direct the integration of a loxP-containing circular DNA into a chromosomal loxP site, both in yeast and in mammalian… CONTINUE READING

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