Targeted inactivation of the plastid ndhB gene in tobacco results in an enhanced sensitivity of photosynthesis to moderate stomatal closure.

@article{Horvath2000TargetedIO,
  title={Targeted inactivation of the plastid ndhB gene in tobacco results in an enhanced sensitivity of photosynthesis to moderate stomatal closure.},
  author={Eva M. Horvath and Stefanie Peter and Thierry Jo{\"e}t and Dominique Rumeau and Laurent Cournac and G{\'a}bor V. Horv{\'a}th and T Anthony Kavanagh and Christoph Schaefer and Gilles Peltier and P{\'e}ter Medgyesy},
  journal={Plant physiology},
  year={2000},
  volume={123 4},
  pages={1337-50}
}
The ndh genes encoding for the subunits of NAD(P)H dehydrogenase complex represent the largest family of plastid genes without a clearly defined function. Tobacco (Nicotiana tabacum) plastid transformants were produced in which the ndhB gene was inactivated by replacing it with a mutant version possessing translational stops in the coding region. Western-blot analysis indicated that no functional NAD(P)H dehydrogenase complex can be assembled in the plastid transformants. Chlorophyll… CONTINUE READING
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