Targeted inactivation of mouse RAD52 reduces homologous recombination but not resistance to ionizing radiation.

Abstract

The RAD52 epistasis group is required for recombinational repair of double-strand breaks (DSBs) and shows strong evolutionary conservation. In Saccharomyces cerevisiae, RAD52 is one of the key members in this pathway. Strains with mutations in this gene show strong hypersensitivity to DNA-damaging agents and defects in recombination. Inactivation of the mouse homologue of RAD52 in embryonic stem (ES) cells resulted in a reduced frequency of homologous recombination. Unlike the yeast Scrad52 mutant, MmRAD52(-/-) ES cells were not hypersensitive to agents that induce DSBs. MmRAD52 null mutant mice showed no abnormalities in viability, fertility, and the immune system. These results show that, as in S. cerevisiae, MmRAD52 is involved in recombination, although the repair of DNA damage is not affected upon inactivation, indicating that MmRAD52 may be involved in certain types of DSB repair processes and not in others. The effect of inactivating MmRAD52 suggests the presence of genes functionally related to MmRAD52, which can partly compensate for the absence of MmRad52 protein.

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@article{Rijkers1998TargetedIO, title={Targeted inactivation of mouse RAD52 reduces homologous recombination but not resistance to ionizing radiation.}, author={Tonnie Rijkers and Johannes M W van den Ouweland and Bruno Morolli and Antonius G. Rolink and Willy M Baarends and Petra P H Van Sloun and Paul H. M. Lohman and Albert Pastink}, journal={Molecular and cellular biology}, year={1998}, volume={18 11}, pages={6423-9} }