Targeted genome modification in mice using zinc-finger nucleases.

@article{Carbery2010TargetedGM,
  title={Targeted genome modification in mice using zinc-finger nucleases.},
  author={Iara D Carbery and Diana Ji and A. Katherine Harrington and Victoria Brown and Edward J. Weinstein and Lucy Liaw and Xiaoxia Cui},
  journal={Genetics},
  year={2010},
  volume={186 2},
  pages={451-9}
}
Homologous recombination-based gene targeting using Mus musculus embryonic stem cells has greatly impacted biomedical research. This study presents a powerful new technology for more efficient and less time-consuming gene targeting in mice using embryonic injection of zinc-finger nucleases (ZFNs), which generate site-specific double strand breaks, leading to insertions or deletions via DNA repair by the nonhomologous end joining pathway. Three individual genes, multidrug resistant 1a (Mdr1a… CONTINUE READING
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