Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

@article{Cho2013TargetedGE,
  title={Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease},
  author={Seung Woo Cho and Sojung C. Kim and Jong Min Kim and Jin-Soo Kim},
  journal={Nature Biotechnology},
  year={2013},
  volume={31},
  pages={230-232}
}
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%. 
Highly Influential
This paper has highly influenced 22 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Recent Discussions
This paper has been referenced on Twitter 12 times over the past 90 days. VIEW TWEETS

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 580 extracted citations

References

Publications referenced by this paper.
Showing 1-3 of 3 references

Methods 7, 91, author reply

  • J. S. Kim, H. J. Lee, Carroll, D. Nat
  • 2010

Similar Papers

Loading similar papers…