Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

  title={Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease},
  author={Seung Woo Cho and Sojung C. Kim and Jong Min Kim and Jin-Soo Kim},
  journal={Nature Biotechnology},
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%. 
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  • J. S. Kim, H. J. Lee, Carroll, D. Nat
  • 2010

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