Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly.

@article{Kim2009TargetedGE,
  title={Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly.},
  author={H. J. Kim and H. J. Lee and H. Kim and S. W. Cho and J. Kim},
  journal={Genome research},
  year={2009},
  volume={19 7},
  pages={
          1279-88
        }
}
Broad applications of zinc finger nuclease (ZFN) technology-which allows targeted genome editing-in research, medicine, and biotechnology are hampered by the lack of a convenient, rapid, and publicly available method for the synthesis of functional ZFNs. Here we describe an efficient and easy-to-practice modular-assembly method using publicly available zinc fingers to make ZFNs that can modify the DNA sequences of predetermined genomic sites in human cells. We synthesized and tested hundreds of… Expand
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