Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility.

@article{Dix1996TargetedGD,
  title={Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility.},
  author={David J. Dix and James W Allen and Barbara W. Collins and Chisato Mori and Noriko Nakamura and Patricia Poorman-Allen and Eugenia H. Goulding and E Mitchell Eddy},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 8},
  pages={3264-8}
}
In addition to the five 70-kDa heat shock proteins (HSP70) common to germ cells and somatic tissues of mammals, spermatogenic cells synthesize HSP70-2 during meiosis. To determine if this unique stress protein has a critical role in meiosis, we used gene-targeting techniques to disrupt Hsp70-2 in mice. Male mice homozygous for the mutant allele (Hsp70-2 -/-) did not synthesize HSP70-2, lacked postmeiotic spermatids and mature sperm, and were infertile. However, neither meiosis nor fertility was… CONTINUE READING
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