Targeted gene disruption in Xenopus laevis using CRISPR/Cas9

  title={Targeted gene disruption in Xenopus laevis using CRISPR/Cas9},
  author={Fengqin Wang and Zhaoying Shi and Yan Cui and Xiaogang Guo and Yunbo Shi and Yonglong Chen},
  booktitle={Cell & Bioscience},
Dear Editor To test if the CRISPR/Cas9 system can mediate targeted gene disruption in Xenopus laevis, we targeted ptf1a/p48 and tyrosinase in this species and found that in addition to high sequence disruption efficiency, clear phenotypes were observed in G0 embryos. ptf1a/ p48-targeted X. laevis embryos can mimic Xenopus tropicalis ptf1a/p48-null mutant tadpoles with respect to the loss of pdip expression. Simultaneous disruption of two X. laevis tyrosinase homeologs leads to almost full… CONTINUE READING
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