Targeted gene disruption in Francisella tularensis by group II introns.

  title={Targeted gene disruption in Francisella tularensis by group II introns.},
  author={Stephen Rodriguez and Gregory Davis and Karl Klose},
  volume={49 3},
Francisella tularensis is a highly infectious Gram-negative bacterium that is the causative agent of tularemia. Very little is known about the molecular mechanisms responsible for F. tularensis virulence, in part due to the paucity of genetic tools available for the study of F. tularensis. We have developed a gene knockout system for F. tularensis that utilizes retargeted mobile group II introns, or "targetrons". These targetrons disrupt both single and duplicated target genes at high… CONTINUE READING

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