Targeted gene correction of hprt mutations by 45 base single-stranded oligonucleotides.

@article{Kenner2002TargetedGC,
  title={Targeted gene correction of hprt mutations by 45 base single-stranded oligonucleotides.},
  author={Oliver Kenner and Andrea Reinhilde Kneisel and Juergen Dr Klingler and Britta Bartelt and Guenter Speit and Walther Vogel and Dieter Prof. Dr. Kaufmann},
  journal={Biochemical and biophysical research communications},
  year={2002},
  volume={299 5},
  pages={
          787-92
        }
}
Targeted correction of a single base in a gene of an eucaryotic cell by specific oligonucleotides is a yet controversial technique. Here, we introduce the correction of point mutations in the hypoxanthine-guanine-phosphoribosyl-transferase (HPRT) gene as an additional model system to test targeted gene correction. In human, Hprt mutations cause Lesch-Nyhan syndrome. Using hamster V79 cells, we generated three cell lines with one hprt point mutation each. These cell lines were treated with… CONTINUE READING
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