Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks.

@article{Robert2007TargetedEO,
  title={Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks.},
  author={Val{\'e}rie J.P. Robert and Jean-Louis Bessereau},
  journal={The EMBO journal},
  year={2007},
  volume={26 1},
  pages={170-83}
}
The Drosophila element Mos1 is a class II transposon, which moves by a 'cut-and-paste' mechanism and can be experimentally mobilized in the Caenorhabditis elegans germ line. Here, we triggered the excision of identified Mos1 insertions to create chromosomal breaks at given sites and further manipulate the broken loci. Double-strand break (DSB) repair could be achieved by gene conversion using a transgene containing sequences homologous to the broken chromosomal region as a repair template… CONTINUE READING

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