Targeted disruption of the Dictyostelium myosin essential light chain gene produces cells defective in cytokinesis and morphogenesis.

@article{Chen1995TargetedDO,
  title={Targeted disruption of the Dictyostelium myosin essential light chain gene produces cells defective in cytokinesis and morphogenesis.},
  author={T L Chen and Paweł Kowalczyk and George T. Ho and Rex L. Chisholm},
  journal={Journal of cell science},
  year={1995},
  volume={108 ( Pt 10)},
  pages={3207-18}
}
We have previously demonstrated that the myosin essential light chain (ELC) is required for myosin function in a Dictyostelium cell line, 7-11, in which the expression of ELC was inhibited by antisense RNA overexpression. We have now disrupted the gene encoding the ELC (mlcE) in Dictyostelium by gene targeting. The mlcE- mutants provide a clean genetic background for phenotypic analysis and biochemical characterization by removing complications arising from the residual ELC present in 7-11… CONTINUE READING

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