Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype.

@article{Threadgill1995TargetedDO,
  title={Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype.},
  author={David W Threadgill and Andrzej A Dlugosz and Laura A. Hansen and Tamar Tennenbaum and Ulrike F. Lichti and Della Yee and Christian LaMantia and Tracy L Mourton and Karl Herrup and Raymond C Harris},
  journal={Science},
  year={1995},
  volume={269 5221},
  pages={230-4}
}
Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal… CONTINUE READING