Targeted disruption in murine cells reveals variable requirement for Smad4 in transforming growth factor beta-related signaling.

@article{Sirard2000TargetedDI,
  title={Targeted disruption in murine cells reveals variable requirement for Smad4 in transforming growth factor beta-related signaling.},
  author={Christian Sirard and Seong-Jin Kim and Christine Mirtsos and P Tadich and Pamela A. Hoodless and Annick Iti{\'e} and Robert M Maxson and Jeffrey L. Wrana and Tak W Mak},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 3},
  pages={2063-70}
}
The tumor suppressor gene Smad4 has been proposed to be a common mediator of transforming growth factor beta (TGFbeta)-related signaling pathways. We investigated the role of Smad4 in TGFbeta-related pathways by targeted disruption of its locus in murine cell lines. TGFbeta responses, including growth arrest, induction of the endogenous PAI-1 gene, and other extracellular matrix components, were normal in Smad4-deficient fibroblasts. Assembly of a TGFbeta-induced DNA-binding complex on one of… CONTINUE READING

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