Targeted deletion of the chicken beta-globin regulatory elements reveals a cooperative gene silencing activity.

@article{Wang2005TargetedDO,
  title={Targeted deletion of the chicken beta-globin regulatory elements reveals a cooperative gene silencing activity.},
  author={Jin Wang and Hui Liu and Chi Mei Lin and Mirit I. Aladjem and Elliot M. Epner},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 24},
  pages={23340-8}
}
The chicken beta-globin locus represents a well characterized system to study the role of both proximal and distal regulatory elements in a eukaryotic multigene domain. The function of the chicken beta(A)/epsilon-intergenic enhancer and upstream regulatory elements 5'-HS1 and 5'-HS2 were studied using a gene targeting approach in chicken DT40 cells followed by microcell-mediated chromosome transfer into human erythroleukemia cells (K562). These regulatory elements all repressed expression of… CONTINUE READING