Targeted axon-attached recording with fluorescent patch-clamp pipettes in brain slices

Abstract

Understanding the physiology of axons in the central nervous system requires experimental access to intact axons. This protocol describes how to perform cell-attached recordings from narrow axon fibers (ϕ <1 μm) in acute and cultured brain slice preparations (with a success rate of ∼50%). By using fluorophore-coated glass pipettes and Nipkow disk confocal… (More)
DOI: 10.1038/nprot.2012.061

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