Targeted analysis of protein termini.

  title={Targeted analysis of protein termini.},
  author={Wilma Dormeyer and Shabaz Mohammed and Bas van Breukelen and Jeroen Krijgsveld and Albert J. R. Heck},
  journal={Journal of proteome research},
  volume={6 12},
We describe a targeted analysis of protein isoforms by selective enrichment and identification of in vivo acetylated protein N-termini and protein C-termini. Our method allows the characterization of these protein termini regardless of their annotation in protein databases and requires no chemical derivatization. Using an iterative database search strategy that takes account of the enrichment protocol, 263 IPI annotated and 87 unpredicted acetylated N-termini were identified in the crude… CONTINUE READING

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