Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi).

@article{Hawkins2015TargetedTR,
  title={Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi).},
  author={John Sir Hawkins and Spencer S. Wong and Jason M Peters and Ricardo V. M. Almeida and Lei S. Qi},
  journal={Methods in molecular biology},
  year={2015},
  volume={1311},
  pages={
          349-62
        }
}
Clustered regularly interspersed short palindromic repeats (CRISPR) interference (CRISPRi) is a powerful technology for sequence-specifically repressing gene expression in bacterial cells. CRISPRi requires only a single protein and a custom-designed guide RNA for specific gene targeting. In Escherichia coli, CRISPRi repression efficiency is high (~300-fold), and there are no observable off-target effects. The method can be scaled up as a general strategy for the repression of many genes… CONTINUE READING
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