Targeted Suppression and Knockout of ASCT2 or LAT1 in Epithelial and Mesenchymal Human Liver Cancer Cells Fail to Inhibit Growth

@article{Bothwell2018TargetedSA,
  title={Targeted Suppression and Knockout of ASCT2 or LAT1 in Epithelial and Mesenchymal Human Liver Cancer Cells Fail to Inhibit Growth},
  author={P. Bothwell and Clare Kron and Evan F Wittke and Bradley N Czerniak and B. P. Bode},
  journal={International Journal of Molecular Sciences},
  year={2018},
  volume={19}
}
  • P. Bothwell, Clare Kron, +2 authors B. P. Bode
  • Published 2018
  • Biology, Medicine
  • International Journal of Molecular Sciences
    • Amino acid transporters alanine-serine-cysteine transporter 2 (ASCT2) and L-Type Amino Acid Transporter 1 (LAT1) are coordinately enhanced in human cancers where among other roles, they are thought to drive mechanistic target-of-rapamycin (mTOR) growth signaling. To assess ASCT2 and LAT1 as therapeutic targets, nine unique short hairpin RNA (shRNA) vectors were used to stably suppress transporter expression in human epithelial (Hep3B) and mesenchymal (SK-Hep1) hepatocellular carcinoma (HCC… CONTINUE READING
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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 48 REFERENCES
    The glutamine transporter ASCT2 (SLC1A5) promotes tumor growth independently of the amino acid transporter LAT1 (SLC7A5)
    • 42
    • Highly Influential
    • Open Access
    ASCT2/SLC1A5 controls glutamine uptake and tumour growth in triple-negative basal-like breast cancer
    • 196
    • Open Access
    ASCT2 silencing regulates mammalian target-of-rapamycin growth and survival signaling in human hepatoma cells.
    • 106
    Inducible antisense RNA targeting amino acid transporter ATB0/ASCT2 elicits apoptosis in human hepatoma cells.
    • 63
    • Open Access
    Impact of system L amino acid transporter 1 (LAT1) on proliferation of human ovarian cancer cells: a possible target for combination therapy with anti-proliferative aminopeptidase inhibitors.
    • 66
    Genetic Disruption of the Multifunctional CD98/LAT1 Complex Demonstrates the Key Role of Essential Amino Acid Transport in the Control of mTORC1 and Tumor Growth.
    • 70
    • Open Access
    Deletion of Amino Acid Transporter ASCT2 (SLC1A5) Reveals an Essential Role for Transporters SNAT1 (SLC38A1) and SNAT2 (SLC38A2) to Sustain Glutaminolysis in Cancer Cells*
    • 83
    • Highly Influential
    • Open Access
    Targeting glutamine transport to suppress melanoma cell growth
    • 134
    • Open Access
    Targeting ASCT2‐mediated glutamine uptake blocks prostate cancer growth and tumour development
    • 146
    • Open Access
    Amino acid transporters ASCT2 and LAT1 in cancer: partners in crime?
    • 523