Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA.

@article{Svitashev2015TargetedMP,
  title={Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA.},
  author={Sergei Svitashev and Joshua K. Young and Christine Schwartz and Huirong Gao and S. Carl Falco and A Mark Cigan},
  journal={Plant physiology},
  year={2015},
  volume={169 2},
  pages={
          931-45
        }
}
Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific insertion of a trait gene using clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas)-guide RNA technology are reported in maize. DNA vectors expressing maize codon-optimized Streptococcus pyogenes Cas9 endonuclease and single guide RNAs were cointroduced with or without DNA repair templates into maize immature embryos by biolistic transformation targeting five different genomic… CONTINUE READING

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