Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells.

@article{Chapman2015TargetedGM,
  title={Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells.},
  author={Karen M Chapman and Gerardo A Medrano and Priscilla Jaichander and Jaideep Chaudhary and Alexandra E Waits and Marcelo A. Nobrega and James M. Hotaling and Carole Ober and F. Kent Hamra},
  journal={Cell reports},
  year={2015},
  volume={10 11},
  pages={1828-35}
}
Organisms with targeted genomic modifications are efficiently produced by gene editing in embryos using CRISPR/Cas9 RNA-guided DNA endonuclease. Here, to facilitate germline editing in rats, we used CRISPR/Cas9 to catalyze targeted genomic mutations in rat spermatogonial stem cell cultures. CRISPR/Cas9-modified spermatogonia regenerated spermatogenesis and displayed long-term sperm-forming potential following transplantation into rat testes. Targeted germline mutations in Epsti1 and Erbb3 were… CONTINUE READING