Targeted Chromosomal Translocations and Essential Gene Knockout Using CRISPR/Cas9 Technology in Caenorhabditis elegans.

Abstract

Many genes play essential roles in development and fertility; their disruption leads to growth arrest or sterility. Genetic balancers have been widely used to study essential genes in many organisms. However, it is technically challenging and laborious to generate and maintain the loss-of-function mutations of essential genes. The CRISPR/Cas9 technology has… (More)
DOI: 10.1534/genetics.115.181883

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Cite this paper

@article{Chen2015TargetedCT, title={Targeted Chromosomal Translocations and Essential Gene Knockout Using CRISPR/Cas9 Technology in Caenorhabditis elegans.}, author={Xiangyang Chen and Mu Li and Xuezhu Feng and Shouhong Guang}, journal={Genetics}, year={2015}, volume={201 4}, pages={1295-306} }