Target structures for HIV-1 inactivation by methylene blue and light.

@article{Bachmann1995TargetSF,
  title={Target structures for HIV-1 inactivation by methylene blue and light.},
  author={B Bachmann and Joseph Kn{\"u}ver-Hopf and Bart Lambrecht and Harald Mohr},
  journal={Journal of medical virology},
  year={1995},
  volume={47 2},
  pages={172-8}
}
In a photodynamic virus inactivation procedure for human fresh frozen plasma the plasma is exposed to visible light in the presence of 1 microM methylene blue. This procedure is known to inactivate HIV-1 by at least 10(6.32) TCID50/ml within 10 minutes. To elucidate the mechanism of photodynamic inactivation of HIV-1 by methylene blue/light treatment, reverse transcriptase (RT), the HIV-1 associated protein p24, and viral RNA were examined. In the dark, methylene blue up to 10 microM has no… CONTINUE READING