Target gene sequencing to characterize the penicillin G susceptibility of Neisseria meningitidis.

@article{Taha2007TargetGS,
  title={Target gene sequencing to characterize the penicillin G susceptibility of Neisseria meningitidis.},
  author={Muhamed-Kkeir Taha and Julio Alberto V{\'a}zquez and Eva Hong and D{\'e}sir{\'e}e E Bennett and Sophie Bertrand and Suzana Bukovski and Mary T Cafferkey and Françoise Carion and Jens J\orgen E Christensen and Mathew A. Diggle and Giles F. S. Edwards and Roc{\'i}o Enr{\'i}quez and C. Victor W. Fazio and Matthias Frosch and Sigrid Heuberger and Steen Hoffmann and Keith A. Jolley and Marcin Kadlubowski and Amel K{\'e}chrid and Konstantinos Kesanopoulos and Paula Kriz and Lotte Lambertsen and Ileanna Levenet and Martin Mus{\'i}lek and Metka Paragi and Aouatef Saguer and Anna Skoczyńska and Paola Stefanelli and Sara Thulin and Georgina Tzanakaki and Magnus Unemo and U. Vogel and Maria Leticia Zarantonelli},
  journal={Antimicrobial agents and chemotherapy},
  year={2007},
  volume={51 8},
  pages={2784-92}
}
Clinical isolates of Neisseria meningitidis with reduced susceptibility to penicillin G (intermediate isolates, Pen(I)) harbor alterations in the penA gene encoding the penicillin binding protein 2 (PBP2). A 402-bp DNA fragment in the 3' half of penA was sequenced from a collection of 1,670 meningococcal clinical isolates from 22 countries that spanned 60 years. Phenotyping, genotyping, and the determination of MICs of penicillin G were also performed. A total of 139 different penA alleles were… CONTINUE READING

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