Target frequency and integration pattern for insertion and replacement vectors in embryonic stem cells.

@article{Hasty1991TargetFA,
  title={Target frequency and integration pattern for insertion and replacement vectors in embryonic stem cells.},
  author={Paul Hasty and J A Rivera-P{\'e}rez and C Chang and Allan Bradley},
  journal={Molecular and cellular biology},
  year={1991},
  volume={11 9},
  pages={4509-17}
}
Gene targeting has been used to direct mutations into specific chromosomal loci in murine embryonic stem (ES) cells. The altered locus can be studied in vivo with chimeras and, if the mutated cells contribute to the germ line, in their offspring. Although homologous recombination is the basis for the widely used gene targeting techniques, to date, the mechanism of homologous recombination between a vector and the chromosomal target in mammalian cells is essentially unknown. Here we look at the… CONTINUE READING

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Inactivating the P2microglobulin locus in mouse embryonic stem cells by homologous recombination

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  • Proc. Natl. Acad. Sci. USA
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