Target for standard Thai PCR assay identical in 12 white spot syndrome virus (WSSV) types that differ in DNA multiple repeat length.

@article{Kiatpathomchai2005TargetFS,
  title={Target for standard Thai PCR assay identical in 12 white spot syndrome virus (WSSV) types that differ in DNA multiple repeat length.},
  author={Wansika Kiatpathomchai and Attaporn Taweetungtragoon and Karnyupha Jittivadhana and Chainarong Wongteerasupaya and Vichai Boonsaeng and Timothy William Flegel},
  journal={Journal of virological methods},
  year={2005},
  volume={130 1-2},
  pages={79-82}
}
A Thai PCR detection method (WSSV-232) yielding a 232 bp amplicon has been used for detection of white spot syndrome virus (WSSV) since 1996. It targets ORF 91 in the full sequence of the only Thai WSSV isolate at GenBank (AF369029). At the beginning of 2002, some Thai shrimp farmers complained that ponds stocked with WSSV-232 PCR negative post-larvae (PL) later suffered WSSV disease outbreaks. Although these outbreaks may have resulted from horizontal transmission of WSSV after stocking, it… CONTINUE READING

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