Target-enrichment strategies for next-generation sequencing

@article{Mamanova2010TargetenrichmentSF,
  title={Target-enrichment strategies for next-generation sequencing},
  author={Lira Mamanova and Alison J. Coffey and Carol E. Scott and Iwanka Kozarewa and Emily H Turner and Akash Kumar and Eleanor Howard and Jay A. Shendure and Daniel J Turner},
  journal={Nature Methods},
  year={2010},
  volume={7},
  pages={111-118}
}
We have not yet reached a point at which routine sequencing of large numbers of whole eukaryotic genomes is feasible, and so it is often necessary to select genomic regions of interest and to enrich these regions before sequencing. There are several enrichment approaches, each with unique advantages and disadvantages. Here we describe our experiences with the leading target-enrichment technologies, the optimizations that we have performed and typical results that can be obtained using each. We… Expand

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